Recombinant Human VEGFR3/FLT4 Protein,hFc Tag

Description :
Source Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Tyr25-Ile776.[Uniprot|P35916-1] Molecular Weight The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human VEGF-C, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human VEGFR3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.