Recombinant Human TGF-alpha Protein

Description :
FAQ COA 已发表文献 Transforming Growth Factor-alpha (TGF-) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF- is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects. Product Properties Synonyms Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF Accession P01135 Gene ID 7039 Source E.coli-derived Human TGF-aprotein, Vla40-Ala89 Molecular Weight Approximately5.8 kDa, on SDS-PAGE under reducing conditions. AA Sequence V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95%as determinedby SDS-PAGE Biological Activity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50for this effect is 0.4 ng/mL. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized after extensive dialysis against PBS. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddHO or PBS up to 100 g/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.