Human LAG-1/CCL4L1

Description :
FAQ COA 已发表文献 LAG-1 is CC chemokine that signals through the CCR5 receptor. LAG-1 is identical to MIP-1 (ACT II isotype) except for two amino acid substitutions; arginine for histidine at position 22 and serine for glycine at position 47 of the mature protein. LAG-1 chemoattracts monocytes, and exhibits activity as an HIV-suppressive factor. Product Properties Synonyms CCL4L1 Accession Q8NHW4 GeneID 388372 Source E.coli-derived Human CCL4L1 protein,Ala24-Asn92. Molecular Weight Approximately7.8 kDa AA Sequence APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 105IU/mg. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.