Recombinant Rat C10/CCL6

Description :
FAQ COA 已发表文献 RatC10 belongs to the CC chemokine family and is expressed in myelopoietic bone marrow cultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4. It signals primarily through the CCR1 receptor. C10 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. The C10 contains the four highly conserved cysteine residues present in CC chemokines. The mature protein contains 95 amino acid residues. Recombinant Murine C10 is a 10.7 kDa protein containing 95 amino acid residues. Product Properties Synonyms Small-inducible Cytokine A6 Accession Q68FP3 GeneID 287910 Source E.coli-derived Rat CCL6 protein,Gly22-Ala115. Molecular Weight Approximately 10.4 kDa AA Sequence GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.