Recombinant Human Eotaxin/CCL11

Description :
FAQ COA 已发表文献 Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Human CCL11 is approximately 63 % identical at the amino acid level to murine CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor. Product Properties Synonyms SCYA11 Protein, Human; Small-inducible cytokine A11 Accession P51671 GeneID 6356 Source E.coli-derived human Eotaxin Protein, Gly24-Pro97 Molecular Weight Approximately 8.4 kDa. AA Sequence GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.