Recombinant Porcine IL-1RA

Description :
FAQ COA 已发表文献 IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptors (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 bothin vitroandin vivo.Human, mouse and rat IL-1ra all share approximately 80% homology with porcine IL-1ra. Product Properties Synonyms DIRA; ICIL-1ra; IL1F3 Accession Q29056 GeneID 397499 Source E.coli-derivedPorcine IL-1RA, His26-Gln177. Molecular Weight Approximately 17.1 kDa. AA Sequence HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by inhibiting IL-1-dependent proliferation of murine D10S cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 104IU/mg in the presence of 75 pg/mLrPoIL-1.Fully biologically active when compared to standard. Endotoxin 0.1EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 1 PBS, pH 7.4, 1 mM DTT. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.