Recombinant Mouse EPO/erythropoietin Protein

Description :
Synonyms EPO; Erythropoietin; EP; MVCD2; Epoetin Source HEK293 Cells Molecular Weight The recombinant mouse Epo predicts a molecular mass of 18.6 kDa. AA Sequence NP_031968.1((Met1-Arg192) Biological Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50for this effect is typically 2-10 ng/mL. Purity > 95% by SDS-PAGE. Endotoxin Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C.