Recombinant Rat Prolactin

Description :
FAQ COA 已发表文献 Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The major form of PRL is a 23 kDa monomeric protein, splice variants of 14, 16, and 22kDa have been identified. PRL has also been found to be glycosylated, phosphorylated, dimerized, and polymerized. Glycosylation, phosphorylation, dimerization, or polymerization of PRL result in lower activity. Rat Prolactin shares 77 % ~ 90 % a.a. sequence identity with human and mouse Prolactin. There are mainly three different forms of Prolactin in regard to size: little Prolactin, big Prolactin and big big Prolactin. Little Prolactin is the predominant form. Although often associated with human milk production, Prolactin plays a wide range of other roles in both humans and other verterbrates. Product Properties Synonyms Mammotropin, Luterotropic hormone, Lutetropin Accession P01237 GeneID 24683 Source E.coli-derived rat Prolactin protein, Leu30-Cys226. Molecular Weight Approximately 22.6 kDa. AA Sequence LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 106IU/mg. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.