Recombinant Biotinylated Human Notch 1 Protein,His-Avi Tag

Description :
Source Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala19-Gln526.[Uniprot :P46531] Molecular Weight The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR DataHuman DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 M as determined in SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.