Recombinant Human IL-22R alpha 1 Protein,hFc Tag

Description :
Source Recombinant Human IL-22R alpha 1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains His16-Thr228.[Uniprot|Q8N6P7] Molecular Weight The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC Activity ELISA Data 1:Immobilized Human IL-22R alpha 1, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-IL-22R alpha 1 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. ELISA Data 2:Immobilized Human IL-22, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.