Recombinant Mouse Midkine

Description :
FAQ COA 已发表文献 Midkine (MK) and its functionally-related protein pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK).MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development.MK chemoattracts embryonic neurons, neutrophils and macrophages, and exerts angiogenic, growth and survival activities during tumorgenesis. Recombinant Mouse Midkine is a 13.2 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds. Product Properties Synonyms MK, NEGF-2 Accession P12025 GeneID 17242 Source E.coli-derived Mouse Midkine, Vla21-Asp140. Molecular Weight Approximately 13.2 kDa. AA Sequence VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.Fully biologically active when compared to standard. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 2 PBS, pH7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.