Recombinant Human BD-3 重组人β防御素3

Description :
FAQ COA 已发表文献 Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. -Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human -defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because -defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Product Properties Synonyms BD-3 Protein, DEFB-3 Protein, DEFB103 Protein,DEFB3 Protein,BD-3 Protein, BD3 Protein, BP-3 Protein, BP3 Protein Accession P81534 GeneID 55894 Source E.coli-derived human Beta-defensin 3 protein, Gly23-Lys67 Molecular Weight Approximately 5.2 kDa. AA Sequence GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by anti-microbial activity against E.coli. is less than 30 g/ml, corresponding to a specific activity of > 33.3 IU/mg. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.