Recombinant Rat SDF-1α/CXCL12α Protein

Description :
FAQ COA 已发表文献 CXCL12 is belonging to the CXC chemokine family. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils.Contrast to SDF-1, SDF-1is shorter by four amino acids at the C-terminal tail. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12 shares approximately 96% amino acid sequence identity with human CXCL12. SDF-1 is highly conserved between species, rat CXCL12 shares approximately 96% amino acid sequence identity with human CXCL12. Product Properties Synonyms CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha, SDF1, SDF1a, SDF1B Accession Q9QZD1 GeneID 24772 Source E.coli-derived rat SDF-1/CXCL12 protein, Lys22-Lys89. Molecular Weight Approximately 7.9 kDa. AA Sequence KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.