Recombinant Rat LIX/CXCL5

Description :
FAQ COA 已发表文献 CXCL5 (epithelial neutrophil-activating peptide-78) is a member of a proangiogenic subgroup of the CXC-type chemokine family of small, secreted proteins. Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Full length CXCL5 (78aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74aa) and ENA-70 (70aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5 which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. CXCL5 targeted therapy would prevent not only the development of type II diabetes in obese subjects, but also several other obesity-related co morbidities. Product Properties Synonyms chemokine (C-C motif) ligand 5, C-X-C motif chemokine 5, CXCL5, CXCL5/ENA-78, Cytokine LIX, ENA-78, Neutrophil-activating peptide ENA-78, neutrophil-activating protein 78, SCYB5ENA78, Small-inducible Cytokine B5 Accession P97885 GeneID 60665 Source E.coli-derived rat LIX/CXCL5 protein, Ala38-Gln130. Molecular Weight Approximately 10.1 kDa. AA Sequence APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 2 PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.