Recombinant Rat IP-10/CXCL10 重组大鼠 IP-10/CXCL10

Description :
FAQ COA 已发表文献 CXC motif chemokine 10 (CXCL10) also known as interferon -induced protein 10kDa (IP-10) or small-inducible cytokine B10 is a cytokine belonging to the CXC chemokine family. The rhesus macaque IP-10 shares 69 %, 95 %, 74 % a.a. sequence identity with human, mouse, rat IP-10. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated Tlymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect.CXCL10 is also recognized as a biomarker that predicts severity of various diseases. Product Properties Synonyms Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1 Accession P48973 GeneID 245920 Source E.coli-derived rat IP-10/CXCL10 protein, Ile22-Pro98. Molecular Weight Approximately 8.7 kDa. AA Sequence IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.Fully biologically active when compared to standard. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.