Recombinant Mouse SDF-1α/CXCL12α Protein

Description :
FAQ COA 已发表文献 CXCL12 is belonging to the CXC chemokine family. SDF_x005f_x001e_1 alpha and SDF_x005f_x001e_1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 is highly conserved between species, mouse CXCL12 shares approximately 93% amino acid sequence identity with human CXCL12. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils.Contrast to SDF-1, SDF-1is shorter by four amino acids at the C-terminal tail. Product Properties Synonyms CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha, SDF1, SDF1a, SDF1B, TLSF, TPAR1 Accession P40224 GeneID 20315 Source E.coli-derived mouse SDF-1/CXCL12 protein, Lys22-Lys89. Molecular Weight Approximately 8.0 kDa. AA Sequence KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.