Recombinant Mouse PF-4/CXCL4

Description :
FAQ COA 已发表文献 CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequenceidentity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain.CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling.Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer.Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration. Product Properties Synonyms chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4 Accession Q9Z126 GeneID 56744 Source E.coli-derived mouse PF-4/CXCL4 protein, Val30-Ser105. Molecular Weight Approximately 8.2 kDa. AA Sequence VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.