Recombinant Mouse MIP-2/CXCL2

Description :
FAQ COA 已发表文献 CXCL2, also named MIP-2, is belonging to the CXC chemokine family.The protein sequence of mouse CXCL2 shows approximately 63% identity to that of mouse KC, another mouse alpha chemokine whose expression is induced by PDGF.The amino acid sequence of mouse CXCL2 is 60 % identical to human CXCL2. This chemokine is secreted from a mouse macrophage cell line. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is a part of chemokine superfamily, which encodes secretory proteins involved in immune regulation and inflammation. CXCL2, a critical chemokine for neutrophils, has been shown to be produced in the rat intestine in response to platelet-activating factor (PAF) and to mediate intestinal inflammation and injury. Product Properties Synonyms chemokine (C-X-C motif) ligand 2, CINC-2a, CINC3, C-X-C Motif Chemokine 2, CXCL2, GRO beta, GRO2, GROB, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, MGSA beta, MGSA-beta, MIP2, SCYB2 Accession P10889 GeneID 20310 Source E.coli-derived mouse MIP-2/CXCL2protein, Ala28-Asn100. Molecular Weight Approximately 7.8kDa. AA Sequence AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml.Fully biologically active when compared to standard. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in20 mM PB, pH 7.4, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.