Recombinant Human NT-3

Description :
FAQ COA 已发表文献 Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer. Product Properties Synonyms HDNF ; NGF-2 ; NGF2 ; NT-3; NT3 Accession P20783-1 Source E. coli-derivedhuman NT-3protein, Tyr139-Thr257 Molecular Weight Approximately 13.8 kDa. AA Sequence YA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 90% by SDS-PAGE. Biological Activity Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 g/mL(100 L/well) can bind human TrkB-Fch. The EC50of human TrkB-Fch is 120-350 ng/mL. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solutionin PBS, pH 5.2. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.