Recombinant Human Follistatin

Description :
FAQ COA 已发表文献 Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a highaffinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced Follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS288) and 315 aa residues (FS315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS315 composed of 300 aa residues.Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Product Properties Synonyms FS; FSActivin-binding protein; FST Accession P19883 GeneID 10468 Source E.coli-derived Human Follistatin, Gly30-Asn317. Molecular Weight Approximately 31.5 kDa. AA Sequence GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Testing in Progress. Endotoxin Formulation Lyophilized from a 0.2 mm filtered concentrated solution in 20mM Tris-HCl, pH 8.5, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.