Recombinant Human Epigen

Description :
FAQ COA 已发表文献 Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, and heart, as well as in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human Epigen is a 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF-homologous portion of the Epigen precursor. Product Properties Synonyms EPG, Epithelial Mitogen Accession Q6UW88 GeneID 255324 Source E.coli-derived Human Epigen protein,Ala24-Ala104. Molecular Weight Approximately 7.9 kDa. AA Sequence AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/mL, corresponding to a specific activity of > 3.3 103IU/mg. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.