Recombinant Human CYR61

Description :
FAQ COA 已发表文献 Cyr61, also known as CCN1,is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration. Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP typeI domain, and a cysteine knot domain. Mature human Cyr61 shares 93%amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2,3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans. Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation. Plasmin cleavage of ECM-bound Cyr61 releases a 28kDa N-terminal fragment which retains the ability to promote endothelial cell migration. Cyr61 exhibits both tumorigenic and tumor suppressor properties. Product Properties Synonyms CCN1, Cysteine-rich Angiogenic Inducer 61, GIG1, IGFBP-10 Accession O00622 GeneID 3491 Source E.coli-derived Human CYR61 protein,Thr25-Asp381. Molecular Weight Approximately 39.4 kDa AA Sequence TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 g/mL, corresponding to a specific activity of > 330 IU/mg. Endotoxin Formulation Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.