Recombinant Mouse LIGHT/TNFSF14

Description :
FAQ COA 已发表文献 LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity.LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Product Properties Synonyms CD258HVEM-L Accession Q9QYH9 GeneID 50930 Source E.coli-derived Mouse LIGHT/TNFSF14, Asp72-Val239. Molecular Weight Approximately 18.4 kDa. AA Sequence DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95 % by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cytotoxicity assay using human HT-29 cells is less than 2 g/mL, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-.Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAc to a concentration of 0.1-1.0 mg/mL.Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.