Recombinant Rhesus TNF-α/TNFSF2

Description :
FAQ COA 已发表文献 Tumor necrosis factor alpha (TNF-), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages.TNF receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-B and the kinase, JNK. Human TNF- shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-. TNF- occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF- in tumour proliferation, migration, invasion and angiogenesis. Product Properties Synonyms TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin Accession P48094 GeneID 715467 Source E.coli-derived Rhesus Macaque TNF-/TNFSF2, Val77-Leu233. Molecular Weight Approximately 17.3 kDa. AA Sequence VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95 % by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 107IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.