Product Name :
Recombinant Mouse TRAIL/TNFSF10
Description :
FAQ COA 已发表文献 TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33-35kDa typeII transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10. Mouse TRAIL cDNA encodes a 17amino acid (aa) N-terminal intracellular domain, a 20aa transmembrane domain and a 253aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer. Unlike other TNF family members, a zinc ion complexed by human Cys230 (mouse Cys240) of each of the three monomers is critical for structural stability. Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells. Accordingly, TRAIL is suggested to have a role in tumor surveillance. Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases. In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin. TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells. Product Properties Synonyms CD253; Protein TRAIL; TL2 Accession P50592 GeneID 22035 Source E.coli-derived Mouse TRAIL/TNFSF10, Pro118-Asn291. Molecular Weight Approximately 20.2 kDa. AA Sequence MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95 % by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 106IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.
Storage :
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