Recombinant HBsAg-preS2

Description :
FAQ COA 已发表文献 The large envelope protein exists in two topological conformations, one which is termed ‘external’ or Le-HBsAg and the other ‘internal’ or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. Product Properties Synonyms Middle surface antigen,PreS2. Accession Q5F4I9 GeneID 10407 Source E.coli-derived HBsAg-preS2 Molecular Weight Approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 55 amino acidswith an N-terminal Met AA Sequence MQWNSTTFHQ ALLDPKVRGL YFPAGGSSSG TVNPVPTTAS PISSIFSRTG DPAPN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity Data is not available. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.