Recombinant HBsAg-preS1 Protein

Description :
FAQ COA 已发表文献 Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1. Product Properties Synonyms L glycoproteinL-HBsAgLarge S proteinLarge surface proteinMajor surface antigen. Accession Q1PD03 GeneID 10407 Source E.coli-derived HBsAg-preS1 Molecular Weight Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acidswith an N-terminal Met AA Sequence MGGWSSKPRQ GMGTNLSVPN PLGFFPDHQL DPAFGANSNN PDWDFNPNKD HWPEAHQVGA GAFGPGFTPP HGGLLGWSPQ AQGILTTVPV APPPASTNRQ SGRQPTPISP PLRDSHPQA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.