Recombinant Human FGF-21 重组人成纤维细胞生长因子21

Description :
FAQ COA 已发表文献 Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene. Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells. The c-DNA for FGF-21 predicts a 209aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region. FGF21 is a metabolic regulator that provides efficient and durable glycemic and lipid control in various animal models. And the FGF21 is a liver-derived endocrine factor that stimulates glucose uptake in adipocytes. Pharmacologic studies show that FGF21 has broad metabolic actions in obese rodents and primates that include enhancing insulin sensitivity, decreasing triglyceride concentrations, and causing weight loss. Product Properties Synonyms FGF21, FGF-21, fibroblast growth factor 21 Accession Q9NSA1 GeneID 26291 Source E.coli-derived human FGF-21protein, His29-Ser209. Molecular Weight Approximately 19.4kDa. AA Sequence HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >96% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 g/ml, corresponding to a specific activity of > 2.0 103IU/mg in the presence of 5 g/ml of recombinant mouse Klotho- and 10 g/ml of heparin.Fully biologically active when compared to standard. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.