Recombinant Human IL-23 Protein

Description :
Synonyms Interleukin-23 Source Sf 21 (baculovirus) Molecular Weight Apparent molecular mass of 65 kDa, 43-45 kDa and 20 kDa in SDS-PAGE under reducing conditions. AA Sequence Human IL-23 p40Ile23-Ser328&Human IL-23 p19(Ala21-Pro189, Accession # AAG37232) Biological Activity Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50for this effect is 0.05-0.3 ng/mL.. Purity > 95% by SDS-PAGE. Endotoxin Formulation Lyophilized from 0.2 m filtered concentrated sterile solution in 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.