Recombinant Human FGF-12

Description :
FAQ COA 已发表文献 Fibroblast growth factor 12(FGF12) is a fibroblast growth factor homologous factor, a subset of the FGF superfamily.Human FGF-12 is synthesized as a 243 aa protein. In human Vascular smooth muscle cells (VSMCs), FGF12 expression was inhibited at the transcriptional level by platelet-derived growth factor-BB. FGF12 inhibited cell proliferation through the p53 pathway and upregulated the key factors involved in VSMC lineage differentiation, such as myocardin and serum response factor.In addition, FGF12 and other FGF11 subfamily members do not activate any fibroblast growth factor receptors (FGFRs), although they can bind to heparin with high affinity like other FGFs. FGF12 has structural similarity with FGF1 and FGF2, in that it lacks a classical signal sequence and con-tains a nuclear localization signal, resulting in the accu-mulation of FGF12 in the nucleus without any release from cells. Product Properties Synonyms FGF12, FGF-12, FGF12B, FHF1, FHF-1, fibroblast growth factor 12, fibroblast growth factor 12B Accession NP_004104 GeneID 2257 Source E.coli-derived human FGF-12 protein, Met1-Thr181. Molecular Weight Approximately 20.5kDa. AA Sequence MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity was determined by its binding ability in a functional ELISA. Immobilizedrecombinant humanFGF R4/Fc Chimera at 5 g/mL (100 L/well) can bindrecombinant humanFGF-12 with a linear range of 1.6-100 ng/mL. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH7.4, with 1 mM DTT. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.