ecombinant Human Growth Hormone (Human GH)

Description :
FAQ COA 已发表文献 Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones – GH-releasing hormone (GHRH) and somatostatin (SST) – as well as by signals from the periphery – ghrelin and leptin . GH stimulates the liver and other tissues to produce IGF-1, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-1. GH, directly or indirectly via IGF-1, can act on B cells, Tcells, NKcells, macrophages and neutrophils to exert immunomodulatory activities. Futhermore, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis. Product Properties Synonyms GH1, GH, GHN, GH-N, hGH-N,Pituitary Growth Hormone, Growth Hormone 1, Somatotropin Accession P01241 GeneID 2688 Source E.coli-derived Human GH, Phe27-Phe217. Molecular Weight Approximately 22kDa. AA Sequence FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 107IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution with 20mM PB, pH 7.0, with 3% Mannitol. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.