Recombinant Human Leptin Protein

Description :
FAQ COA 已发表文献 Leptin is aprotein product of the mouseobesegene . It modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. As a signal molecule with growth factor characteristics, Leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis. Product Properties Synonyms Obese Protein, Obesity Factor Accession P41159 GeneID 3952 Source E.coli-derived Human Leptin, Val22-Cys167. Molecular Weight Approximately 16.0 kDa. Tag None Purity > 95% by SDS-PAGE. Biological Activity Immobilized Human Leptin Antibody at 3 g/mL (100 L/well) can bind Human Leptin , the EC50of Human Leptin is 174-246 pg/mL. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 1 PBS, pH 7.4, 1%BSA. Reconstitution 1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1%BSA to a concentration of 0.1-1.0 mg/mL. 2.Stock solutions should be apportioned into working aliquots and stored at -20 C. 3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1%BSA, 10%FBS, 5%HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum.