Recombinant Human Bcl-xL Protein

Description :
FAQ COA 已发表文献 Bcl-xLis a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-xLis an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xLis present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xLcontains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xLmissing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-xLappears to be mediated through binding the BH3region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence. Product Properties Synonyms Apoptosis regulator Bcl-X Accession Q07817 GeneID 598 Source E.coli-derivedHuman Bcl-2, Ser2-Arg212. Molecular Weight Approximately 23.7kDa. AA Sequence SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEGT ESEMETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIAAWMA TYLNDHLEPW IQENGGWDTF VELYGNNAAA ESRKGQERFN R Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97%by SDS-PAGE and HPLC analyses. Biological Activity Test in Process. Endotoxin Less than 0.1 EU/g of rHuBcl-xL as determined by LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 5% Trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.