Recombinant Human Bcl-2

Description :
FAQ COA 已发表文献 Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability. Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence. Product Properties Synonyms PPP1R50 Protein Accession P10415 GeneID 596 Source E.coli-derived Human Bcl-2, Ala2-Asp211. Molecular Weight Approximately 23.2 kDa. AA Sequence AHAGRTGYDN REIVMKYIHY KLSQRGYEWD AGDVGAAPPG AAPAPGIFSS QPGHTPHPAA SRDPVARTSP LQTPAAPGAA AGPALSPVPP VVHLTLRQAG DDFSRRYRRD FAEMSSQLHL TPFTARGRFA TVVEELFRDG VNWGRIVAFF EFGGVMCVES VNREMSPLVD NIALWMTEYL NRHLHTWIQD NGGWDAFVEL YGPSMRPLFD Tag None Physical Appearance Sterile liquid. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Test in Process. Endotoxin Less than 0.1 EU/g of rHuBcl-2 as determined by LAL method. Formulation 0.2 m filtered concentrated solution in 25 mM Tris-HCl, pH 8.0, 100mM NaCl, 1 mM DTT, 30% Glycerol, with Tween-80. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.