Recombinant Human TARC/CCL17 Protein

Description :
FAQ COA 已发表文献 CCL17,also known as thymus and activation-related chemokine (TARC), a small cytokine belonging to the C-C chemokine family. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 has been shown to be chemotactic for Tcell lines but not monocytes or neutrophils. CCL17 wasidentified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. Product Properties Synonyms MGC138273,SCYA17,SCYA17MGC138271 Accession Q92583 GeneID 6361 Source E.coli-derivedHuman TARC/CCL17, Ala24-Ser93. Molecular Weight Approximately 8.1kDa. AA Sequence ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97%by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.