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Product Name :
Recombinant Protein A/G(Freeze-Dried)重组蛋白A/G (冻干)

Description :
FAQ COA 已发表文献 天然蛋白AProtein A是一种发现于金黄色葡萄球菌的细胞壁表面蛋白天然蛋白GProtein G是一种分离自G型或C型链球菌属的细胞表面蛋白二者功能相似主要通过与免疫球蛋白Ig的Fc区相互作用可结合大多数哺乳动物的IgG然而两者结合特异性上有所不同详细见附录1蛋白AG和L对不同物种Ig的结合能力总表如蛋白G对人IgG3大鼠IgG2a绵羊IgG1具有很高的亲和力但蛋白A对这些Ig结合力很弱蛋白A蛋白G常共价偶联在固体载体如琼脂糖珠或丙烯酸树脂小珠上直接用以做免疫沉淀IP或者免疫共沉淀Co-IP来进行蛋白功能相关研究或者直接装在层析柱上来纯化单克隆或者多克隆抗体 本品在大肠杆菌中产生重组蛋白A/G由7个IgG 结合结构域 EDABC-C1C3组成对应于5个蛋白A的IgG结合区和2个蛋白G的IgG结构域重组蛋白A/G的结合能力比单独的蛋白A或蛋白G更广重组蛋白A/G中细胞壁结合区细胞膜结合区和白蛋白结合区已去除减少了交叉反应和非特异性结合确保IgG的最大特异性结合 产品性质 来源 E.coli 分子量 Approximately47.7 kDa 氨基酸序列 NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTE 280 nm 处1个单位DO的浓度 1.741 mg/ml 外观 无菌过滤的白色冻干粉末 标签 无 > 97% by SDS-PAGE andHPLC analyses 内毒素水平 0.1 EU/g of protein bytheLAL method 配方 无添加剂冻干 复溶 蒸馏水或盐水溶解

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