Recombinant Mouse LIF Protein重组小鼠白血病抑制因子

Description :
FAQ COA 已发表文献 Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. Mature human LIF shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. LIF is multifunctional cytokines with many similar activities. LIF is structurally and functionally related to another cytokine, Oncostatin M (OSM), that binds to the high-affinity LIF receptor but not to the low-affinity LIF receptor. While some of the actions of LIF on neurons resemble those of CNTF, LIF also has broad actions outside of the nervous system that in many cases mimic those of interleukin-6 (IL-6). Product Properties Synonyms CDF,DIA,Differentiation-stimulating Factor,D Factor,Emfilermin,HILDA,Melanoma-derived LPL Inhibitor,MLPLI Accession P09056 GeneID 16878 Source E.coli-derived mouse LIF, Ser24-Phe203. Molecular Weight Approximately 19.9 kDa. AA Sequence SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98%by SDS-PAGEand HPLC analyses. Biological Activity The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of recombinant mouseLIF in this assay is 0.01 ng/mL. The specific activity of > 1.0 108IU/mg, where 50 units is defined as the amount of recombinant mouseLIF required to induce differentiation in 50%of the M1 colonies in 1 ml agar cultures. Fully biologically active when compared to standard. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in20 mM PB, pH 7.4, with 0.02%TWEEN 20. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.