Recombinant Rat CNTF重组大鼠睫状神经营养因子

Description :
FAQ COA 已发表文献 Ciliary neurotrophic factor (CNTF) is one of a small number of proteins with neurotrophic activities distinct from nerve growth factor (NGF).The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence.Recombinant human CNTF containing 200 amino acids and it shares 82% and 83% a.a. sequence identity with mouse and rat CNTF. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. CNTF is structurally related to IL-6, IL-11, LIF and OSM.Among factors belonging to the CNTF family, CNTF, leukemia inhibitory factor, cardiotrophin-1, and oncostatin M induced a strong promyelinating effect. Product Properties Synonyms ciliary neurotrophic factor, CNTF,HCNTF Accession P20294 GeneID 25707 Source E.coli-derived rat CNTF, Ala2-Met200. Molecular Weight Approximately 22.7 kDa. AA Sequence AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/mL, corresponding to a specific activity of > 3.3 104IU/mg.Fully biologically active when compared to standard. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in2 PBS, pH 7.4, 2% trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.