Recombinant Mouse IGF-1

Description :
FAQ COA 已发表文献 Insulin-like Growth Factor I (IGF-I), is the dominant effector of Growth Hormone (GH) and is structurally homologous to ProInsulin.The 7.6 kDa mature IGFI is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I shares 94% and 96%amino acid (aa)sequence identity with the mouse and rat orthologs, respectively. The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-I has been shown to enhance neuronal survival and inhibit apoptosis. IGF-1 promote the proliferation of multiple myeloma (MM) cells and protect them against dexamethasone (Dex)-induced apoptosis. Product Properties Synonyms Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF Accession P05017 GeneID 16000 Source E.coli-derived mouse IGF-1 protein, Gly49-Ala118. Molecular Weight Approximately 7.7 kDa. AA Sequence GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 105IU/mg.Fully biologically active when compared to standard. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.