Recombinant Rat VEGF164

Description :
FAQ COA 已发表文献 Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. Five VEGF isoforms are generated as a result of alternative splicing from a single VEGF gene. These isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. Mouse VEGF164shares 97%aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. During pathological neovascularization, both the absolute and relative expression levels for VEGF164 increased to a greater degree than during physiological neovascularization. Product Properties Synonyms Vascular Endothelial Growth Factor Isoform 164 Accession P16612 Unigene 83785 Source Yeast-derived rat VEGF164 protein, Ala27-Arg190 (N115K), with Met at N-terminus. Molecular Weight Approximately 25.7kDa in SDS-PAGE under non-reducing conditions. AA Sequence MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and 90% by SEC-HPLC analyses. Biological Activity Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50for this effect is 0.753.75 ng/mL.Fully biologically active when compared to standard. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.