Recombinant Rat PDGF-BB

Description :
FAQ COA 已发表文献 Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma.In vitro, PDGF-BB stimulated MAP kinase activity and cell motility of isolated lymphatic endothelial cells. In vivo, PDGF-BB potently induced growth of lymphatic vessels. Expression of PDGF-BB in murine fibrosarcoma cells induced tumor lymphangiogenesis, leading to enhanced metastasis in lymph nodes.The PDGF signaling system contributes to and vascular remodeling.Growth factors such asPDGF exert potent effects on wound healing including the regeneration of tooth-supporting structures.Some research reports findings in this chronic myocardial infarction model were that bFGF, PDGF-BB and their combination all had a more pronounced angiogenic effect on the arteriolar than the capillary level. bFGF stimulated both capillary and arteriolar growth while PDGF-BB preferentially stimulated arterioles. Product Properties Synonyms Becaplermin, ODGF, PDGF-2,PDGFBB,PDGF-BB Accession Q05028 GeneID 24628 Source E.coli-derived rat PDGF-BB protein, Ser74-Thr182. Molecular Weight Approximately 24.4 kDa. AA Sequence SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 105IU/mg.Fully biologically active when compared to standard. Endotoxin 0.1EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 6.9. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.