Recombinant Rat PDGF-AA

Description :
FAQ COA 已发表文献 PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin regeneration. PDGF has been suggested to have a significant role in wound healing. Product Properties Synonyms PDGF1, PDGF-A, PDGFAA, PDGF-AA Accession P28576 GeneID 25266 Source E.coli-derived rat PDGF-AA, Ser87-Arg196, with an N-terminal Met. Molecular Weight Approximately 25.3 kDa. AA Sequence MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98% by SDS-PAGE analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/mL, corresponding to a specific activity of > 2.0 105IU/mg.Fully biologically active when compared to standard. Endotoxin 0.1EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in30% Acetonitrile and 0.1% TFA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.