Recombinant Rat KGF-2/FGF-10

Description :
FAQ COA 已发表文献 Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively.Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively.FGF-10 is a stromal-derived paracrine growth factor considered to be important during embryogenesis. FGF-10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBP through an autocrine/paracrine mechanism. During cardiogenesis, FGF-10 is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (FGF-8), FGF10 promotes the proliferation of these cardiac progenitor cells that form the arterial pole of the heart. Product Properties Synonyms FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 Accession P70492 GeneID 25443 Source E.coli-derived rat FGF-10, Gln37-Ser215. Molecular Weight Approximately 20.0 kDa. AA Sequence QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGEand HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/mL, corresponding to a specific activity of > 8.3 103IU/mg.Fully biologically active when compared to standard. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in20 mM Tris, 500 mM NaCl, pH 7.4, 5% trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.