Recombinant Rat G-CSF

Description :
FAQ COA 已发表文献 Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma.Human G-CSF is 73% identical at the amino acid level to mouseG-CSF and the two proteins show species cross-reactivity.G-CSF receptor was expressed on cardiomyocytes and G-CSF activated the Jak/Stat pathway in cardiomyocytes. The G-CSF treatment did not affect initial infarct size at 3 d but improved cardiac function as early as 1 week after myocardial infarction.And theG-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4. Product Properties Synonyms G-CSF, CSF-3, MGI-1G, GM-CSF beta, MGC45931, Pluripoietin Accession P97712 GeneID 25610 Source E.coli-derived rat G-CSF protein, Ile22-Ile214. Molecular Weight Approximately 21.5 kDa. AA Sequence KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 107IU/mg. Fully biologically active when compared to standard. Endotoxin 0.1EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.