Recombinant Mouse M-CSF Protein

Description :
FAQ COA 已发表文献 Monocyte colony-stimulating factor (M-CSF, CSF-1) is a macrophage lineage-specific growth factor.Full length human M-CSF transcripts encode a 522 amino acid (aa) typeI transmembrane (TM) protein with a 464 aa extracellular region, a 21aa TMdomain, and a 37aa cytoplasmic tail that forms a 140 kDa covalent dimer.The first 223aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M_x005f_x001e_CSF, respectively. M-CSF Induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Whatmore, M-CSF and GM-CSF are mediators involved in regulating the numbers and function of macrophage lineage populations and have been shown to contribute to macrophage heterogeneity. Product Properties Synonyms CSF-1,MGI-IM, MCSF,M-CSF,MGC31930 Accession Q3U4F9 UniGene Mm.795. Source E.coli-derived Mouse M-CSF protein, Lys33-Glu262. Molecular Weight Approximately 52.0kDa. AA Sequence KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 105IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.