Recombinant Human BCA-1/CXCL13

Description :
FAQ COA 已发表文献 The homeostatic chemokine CXCL13 (also called B cell-attracting chemokine 1 [BCA-1] or B-lymphocyte chemoattractant [BLC]) is constitutively expressed in secondary lymphoid tissue and initiates lymphoid neogenesis when expressed aberrantly. Recombinant human CXCL13is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines.CXCL13 is a homeostatic chemokine for lymphocyte homing and positioning within follicles of secondary lymphoid tissues, acting through its cognate receptor, CXCR5. Besides,CXCL13 is a potent chemokine, produced by mature and recently recruited macrophages to sites of inflammation, which has antimicrobial and anti-angiogenic properties. Product Properties Synonyms Angie, B lymphocyte chemoattractant, BCA1, CXC chemokine BLC, Small-inducible cytokine B13, SCYB13 Accession O43927 GeneID 10563 Source E.coli-derived Human BCA-1/CXCL13, Val23-Pro109. Molecular Weight Approximately 10.3 kDa. AA Sequence VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97%by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/mL. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.