Recombinant Rat IL-4 Protein

Description :
FAQ COA 已发表文献 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells.Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively.Itis a potent cytokine produced by T cells and to a lesser extent by tumor-associated natural killer cells, basophils, and mast cells.IL-4 is adifferentiation factor for transforming growth factor-beta secreting Th3.AndIL-4 activates two distinct signalling pathways through tyrosine phosphorylation of Stat6, a signal transducer and activator of transcription, and of a 170K protein called 4PS. Product Properties Synonyms Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra Accession P20096 GeneID 287287 Source E.coli-derived rat IL-4 protein, His23-Ser147. Molecular Weight Approximately 14.0 kDa. AA Sequence HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 106IU/mg. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4, 5% trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.