Recombinant Rat IL-10

Description :
FAQ COA 已发表文献 Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of -helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155.IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer.Mature human IL10 shares 72%86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL10.IL-10, a cytokine produced by CD4+ T lymphocytes belonging to the Th-2 subset, has previously been shown to inhibit the synthesis of IFN-gamma by both T cells and NK cells.The anti-inflammatory actions of IL-10 may be therapeutically useful either directly or through modulation of HO-1 activity. Product Properties Synonyms Interleukin-10,IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF Accession P29456 GeneID 25325 Source E.coli-derived rat IL-10 protein, Ser19-Asn178. Molecular Weight Approximately 18.6 kDa. AA Sequence SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 106IU/mg.Fully biologically active when compared to standard. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.