Recombinant Rat IL-1α

Description :
FAQ COA 已发表文献 Interleukin-1 alpha (IL1a) is also known asIL-1A, IL1,and is a cytokine of the interleukin-1 family.Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines.It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses.IL-1is a major immunoregulatory/proinflammatory cytokine which also affects fibroblast proliferation and function and therefore it was of interest to investigate whether its constitutive expression influences the in vivo tumorigenic potential of transformed fibroblastoid cell lines. Product Properties Synonyms Interleukin-1 alpha, IL-1, BAF, IL-1F1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha Accession P16598 GeneID 24493 Source E.coli-derived rat IL-1alpha protein, Ser115-Ser270. Molecular Weight Approximately 17.8 kDa. AA Sequence SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine D10S cells is less than 0.005 ng/mL, corresponding to a specific activity of > 2.0 108IU/mg. Fully biologically active when compared to standard. Endotoxin EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.