Recombinant Human TPO Protein,HisTag

Description :
FAQ COA 已发表文献 Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis.The 353 amino acid (aa) human TPO precursor is cleaved to yield the 332 aa mature protein. Mature human TPO shares approximately 70% aa sequence homology with mouse and rat TPO. TPO and its receptor (c-Mpl) are the major regulators of megakaryocyte and platelet production and serve a critical and non-redundant role in hematopoietic stem cell (HSC) biology. TPO signals through the Jak-STAT, Ras-Raf-MAPK, and PI3K pathways, and promotes survival, proliferation, and polyploidization in megakaryocytes. The proto-oncogene c- also plays an important role in many of these same processes. Product Properties Synonyms MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, THCYT1, THPO Accession NP_000451.1 Source HEK293 Cells-derived human TPO protein, Ser 22-Gly 353 with His tag at theN-terminus. Molecular Weight Approximately 37.6 kDa. As a result of glycosylation, TPO migrates as an approximately 78.2 kDa band in SDS-PAGE under reducing conditions. AA Sequence spappacdl rvlskllrds hvlhsrlsqc pevhplptpvllpavdfslg ewktqmeetk aqdilgavtl llegvmaarg qlgptclssl lgqlsgqvrlllgalqsllg tqlppqgrttahkdpnaifl sfqhllrgkv rflmlvggst lcvrrappttavpsrtslvl tlnelpnrts glletnftas arttgsgllk wqqgfrakip gllnqtsrsldqipgylnri hellngtrgl fpgpsrrtlg apdissgtsd tgslppnlqp gyspspthpptgqytlfplp ptlptpvvql hpllpdpsap tptptsplln tsythsqnls qeg Tag His Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 90% by SDS-PAGE. Biological Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50for this effect is typically 10-60 ng/mL. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solutionin PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water (400 L) to a concentration of 0.25mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.